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Bench calculators for peptide & solution work

Lab math, shown in full.

Free calculators for molecular weight, molarity, dilutions and units. Every tool shows its formula, a worked example and the sources behind it, so you can check the numbers before you trust them.

12free calculators
12guides explaining the maths
f(x)formula and working on every result
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The toolbox

Calculators, grouped by the job at hand

Results update as you type, and the formula is shown with your own numbers.

Tools index

Sequence tools

Molecular weight, composition and notation for amino-acid sequences.

3 tools
T-01

Peptide Molecular Weight Calculator

Average and monoisotopic molecular weight plus molecular formula for any peptide sequence, with terminal modifications and disulfide bonds.

M = Σ residue masses + M(H₂O) + Δ(modifications)
Open tool Read the guide
T-02

Amino Acid Sequence Analyzer

Composition, net charge at any pH, isoelectric point, GRAVY hydropathy and extinction coefficient for a peptide sequence.

Z = Σ Nᵢ / (1 + 10^(pH − pKaᵢ)) − Σ Nⱼ / (1 + 10^(pKaⱼ − pH))
Open tool Read the guide

Mass & moles

Move between grams, moles and molarity using molar mass.

2 tools
T-05

Molarity Calculator

Solve for mass, volume or molar concentration from the other two values and the molecular weight.

m = c × V × M
Open tool Read the guide

Solutions & dilutions

Plan stock solutions, dilutions and concentration series at the bench.

4 tools
T-07

Concentration Converter

Convert between molar units (M, mM, µM, nM) and mass-per-volume units (mg/mL, µg/mL, % w/v, ppm) using molecular weight.

c_mass (g/L) = c (mol/L) × M (g/mol)
Open tool Read the guide
T-11

Stock Solution Calculator

Mass to weigh for a stock solution, or solvent volume for a weighed amount, with purity correction and aliquot planning.

m_weigh = (c × V × M) / p
Open tool Read the guide

Unit converters

Mass, volume, amount, concentration and temperature units.

3 tools
T-09

Lab Unit Converter

Convert volume, amount of substance, molar concentration, mass concentration and length units used at the bench.

value_target = value × factor_source / factor_target
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Built to be checked

No black boxes

Methodology & sources

The formula, with your numbers

Each result lists the equation and the working, so a misplaced unit is easy to spot before it reaches the bench.

Constants from primary sources

Atomic weights, residue formulas, pKa values and extinction coefficients are taken from IUPAC, NIST and the original papers, and cited on every page.

Shareable and citable

Copy a link that reopens your exact inputs, cite a tool in a methods section, or embed it on a lab wiki or course page.

Guides

How each calculation works

Short explanations with worked examples, common mistakes and references. Useful for teaching, too.

All guides
  1. G-01

    How Peptide Molecular Weight Is Calculated

    Why a peptide weighs less than its amino acids added together, how residue masses work, when to use average or monoisotopic mass, and how terminal groups and disulfides change the result.

  2. G-02

    Amino Acid Properties Explained: Charge, pI, Hydropathy and A280

    What the sequence analyzer reports and how to read it: net charge at a given pH, isoelectric point, GRAVY hydropathy and the extinction coefficient at 280 nm, with the limits of each estimate.

  3. G-03

    Peptide Sequence Notation: One-Letter and Three-Letter Codes

    How peptide sequences are written: direction from N- to C-terminus, the 20 one- and three-letter codes, terminal groups like H- and -NH2, and the ambiguous codes that trip up calculators.

  4. G-04

    Moles and Molar Mass: Converting Between Grams and Moles

    The mole, Avogadro’s number and molar mass explained for bench work, with the conversion n = m / M worked through for peptide-scale quantities.

  5. G-05

    Molarity Explained, With Worked Examples

    What molar concentration means, how to rearrange m = c × V × M to find mass, volume or concentration, and the preparation details that keep a solution at the molarity you calculated.

  6. G-06

    The Dilution Equation (C1V1 = C2V2) and Serial Dilutions

    Why C1V1 = C2V2 works, how to solve it for any unknown, what “1:10” really means, and how to plan serial dilutions that stay accurate at small volumes.

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