Dilution Calculator (C1V1 = C2V2)
Solve C1V1 = C2V2 for any one value, or plan a serial dilution with transfer and diluent volumes for each step.
Topic
Plan stock solutions, dilutions and concentration series at the bench.
Solve C1V1 = C2V2 for any one value, or plan a serial dilution with transfer and diluent volumes for each step.
Convert between molar units (M, mM, µM, nM) and mass-per-volume units (mg/mL, µg/mL, % w/v, ppm) using molecular weight.
Mass to weigh for a stock solution, or solvent volume for a weighed amount, with purity correction and aliquot planning.
Build a printable worksheet of stock and diluent volumes for a list or series of target concentrations.
Why C1V1 = C2V2 works, how to solve it for any unknown, what “1:10” really means, and how to plan serial dilutions that stay accurate at small volumes.
How molar and mass-per-volume concentrations relate, why the molecular weight is the bridge between them, and what percentage and ppm really mean for a solution.
A bench-level walk-through of stock solution preparation: choosing a concentration, correcting for purity and net peptide content, weighing, dissolving, making up to volume, and aliquoting.
How to space concentrations for an experiment (linear, 2-fold, half-log or 10-fold), when to dilute directly or serially, how to keep pipetting volumes accurate, and why the vehicle should stay constant.