Experimental Concentration Worksheet
Build a printable worksheet of stock and diluent volumes for a list or series of target concentrations.
Tubes on worksheet
Working
About this tool
Plan a set of working concentrations from one stock and print it as a bench worksheet. Enter a list of targets or generate a series from a top concentration and a factor. The worksheet shows the stock and diluent volume for every tube, with space for the experiment name, date and initials.
How to use it
- Enter the stock concentration and the final volume for each tube.
- Enter target concentrations as a list, or generate a series.
- Add the experiment details and print, or save as PDF from the print dialog.
Formula f(x)
Each tube is a direct dilution from the stock:
Vstock = Ctarget × Vfinal / Cstock and Vdiluent = Vfinal − Vstock.
Series targets: Ck = Ctop / Fk.
Worked example check your setup
From a 10 mM stock, 1 mL each of 100, 50 and 10 µM needs 10 µL, 5 µL and 1 µL of stock topped up to 1 mL. Volumes under about 2 µL are flagged, because they are hard to pipette accurately. An intermediate dilution is the better choice there.